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Live sample screening at the
AMI Imaging Centre

Zeiss Celldiscoverer 7 high-throughput live-cell screening microscope

The AMI live-cell high-throughput microscopy can be used by researchers to study the behaviour of living cells in real time and in response to a large number of varying experimental conditions. The AMI live-cell screening microscope has the unique ability to not only provide high-detail, sub-cellular spatial information across time, but also provide visiting researchers with exceptionally large datasets containing information on a full range of experimental conditions. Examples of this include drug concentration dilutions, gene induction titrations, and CRISPR-interference construct differences across a 96-well microtitre plate. The automated imaging means that researchers can capture biological processes in high detail from a very large numbers of cells rather than simply relying on low sample numbers.

With temperature and carbon dioxide control, the AMI live-cell screening microscope can also accommodate other types of samples (and holders), such as seeds, blood smears, or developing embryos within a dish. The automation to capture multiple areas of interest will provide visiting researchers with large amounts of biological information that can be used to tease out small responses of the sample. 

Instrument specifications

  • Inverted microscope configuration

  • Auto focus for time-lapse acquisitions of live samples in aqueous media

  • Automatic immersion media control

  • Contrast methods:

    • bright-field transmitted light

    • fluorescence

  • Temperature-, relative humidity, and CO₂-controlled incubation

  • Objectives:​

    • 50× 1.2 NA water-immersion

    • 20× 0.95 NA 

    • 20× 0.7 NA 

  • Light source:

    • Halogen lamp for transmitted light

    • LED for fluorescence​

      • 625nm 

      • 590nm 

      • 567nm 

      • 520nm 

      • 470nm 

      • 420nm 

      • 385nm 

  • Emission filters:​

    • Simultaneous quad-band pass

      • ​410-440nm 

      • 499-529nm 

      • 567-617nm 

      • 659-759nm 

    • Simultaneous tri-band pass

      • 455-489nm 

      • 543-568nm 

      • 608-753nm 

    • ​Simultaneous tri-band pass

      • 410-440nm 

      • 499-529nm 

      • 620-765nm 

  • Axiocam 712 monochrome camera


Instrument capabilities

  • Resolution limit: ~260nm xy; 1800nm z

  • Speed limit: down to 5min for a 96-well microtitre plate

  • Depth: down to 15μm

  • Field of view: dependent on objective

Instrument uses

  • Live-cell imaging

  • Automated stage movement

  • Tiling and panorama (for large fields of view)

  • Long-duration live-cell dynamics and responses

  • Cell migration and movement

  • Organelle tracking

  • Gene expression studies

  • Immune cell response and translocation

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